ABSTRACT
Objective To observe the clinical efficacy of yang-supplementing fire moxibustion in treating chronic obstructive pulmonary disease (COPD) in stable stage. Method Totally 120 COPD patients of yang deficiency type in stable stage were divided into 3 groups by the random number table, a yang-supplementing fire moxibustion group (yang-supplementing group), a dry mild moxibustion group (mild moxibustion group), and a regular Western medication group (regular group), 40 cases in each group, and 30-day treatment was taken as a course. They were treated and observed for a course, followed by a 3-month follow-up study. The pulmonary function indexes, yang-deficiency signs, quality of life, and comprehensive therapeutic efficacy were observed in the three groups. Result After treatment, the pulmonary function indexes, yang deficiency signs, quality of life, and comprehensive therapeutic efficacy were significantly improved in the three groups (P<0.01); the percent of predicted forced expiratory volume in one second (FEV1%) and yang deficiency signs were significantly improved after intervention in the mild moxibustion group (P<0.05); meanwhile, the quality of life was also significantly improved after intervention in the mild moxibustion group and regular group (P<0.01); the therapeutic efficacy of the yang-supplementing group was better than that of the mild moxibustion group and regular group, and the efficacy of the mild moxibustion group was better than that of the regular group. Conclusion Yang-supplementing fire moxibustion can improve the pulmonary function, postpone the progressive decrease of pulmonary function, significantly improve the yang deficiency constitution, enhance the quality of life and comprehensive therapeutic efficacy in treating yang-deficient COPD patients in stable stage.
ABSTRACT
BACKGROUND: Estradiol produces great adverse effects in treatment of postmenopausal osteoporosis.OBJECTIVE: To investigate the effects of small dose of estradiol combined with estradiol on bone tissue of ovariectomized rats.METHODS: A total of 120 healthy female rats were randomly and evenly divided into five groups: sham-operated (only resectionof a small block of para-ovarian fat pad), ovariectomized (without drugs), puerarin, estradiol, and puerarin + estradiol. One weeklater, rats from the puerarin, estradiol and puerarin + estradiol groups were subcutaneously injected with puerarin (50 mg/kg, oncea day), estradiol (200 μg/kg, twice a week), estradiol puerarin (25 mg/kg, once a day) + (100 μg/kg, twice a week), respectively.RESULTS AND CONCLUSION: In the ovariectomized group, rat bone tissue was sparse, bone calcium and phosphorus levelsas well as bone mineral density were significantly lower compared with sham-operated group (P < 0.01). After estradiol and/orpuerain treatment, rat bone tissue morphology was obviously improved, and bone calcium and phosphorus levels and bonemineral density were significantly increased. The above-mentioned indices were similar after small dose of puerarin combinedwith estradiol treatment and high dose of puerarin combined with estradiol treatment. These findings suggest that small dose ofestradiol combined with estradiol treatment yields satisfactory curative effects in treatment of osteoporosis in ovariectomized rats.
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BACKGROUND: Based on the multi-directional differentiation potential,the combined application of proper biological materials and growth factor can repair tissue defect.OBJECTIVE: To observe the growth characteristics of rabbit bone marrow-derived mesenchymal stem cells and their osteogenic potential under the induced condition.DESIGN: A single sample experiment.SETTING: The Department of Histology and Embryology of Youjiang Medical College for Nationalities; the Affiliated Hospital of Youjiang Medical College for Nationalities.MATERIALS: This experiment was conducted at the Department of Histology and Embryology of Youjiang Medical College for Nationalities in June 2004. Male new Zealand rabbits, with a body mass of 2.0 to 2.5 kg,were provided.METHODS: Rabbit bone marrow tissue was extracted. After gradient centrifugation, adherent cells were kept for cell passage. Culture medium was used for inducing culture of osteoblast when the passage was stable (50 mL/L of Dulbecco modified culture medium of bovine serum containing 10 mmol/L of β-sodium glycerophosphate, 10 nmol/L of dexamethasone, 50 mg/L of vitamine C). The culture medium was changed every other day. Osteogenic characteristics of the bone marrow-derived mensenchymal stem cells were observed under an inverted microscope after alkaline phosphatase immunohistological staining, osteonectin and osteopontin immunocytochemical staining.MAIN OUTCOME MEASURES: ① Morphological observation of bone marrow-derived mesenchymal stem cells; ② Osteogenic characteristics of bone marrow-derived mesenchymal stem cells.RESULTS: ① Morphological observation of bone marrow-derived mensenchymal stem cells: Primary rabbit bone marrow-derived mesenchymal stem cells could cover the Petri dish fully in 7 or8 days and could passage stably. Generative cells began to passage in 5 or 6 days. ② Osteogenic characteristics of bone marrow-derived mensenchymal stem cells:Alkaline phosphatase immunohistochemical staining showed a lot of brown particles in the kytoplasm, but no colored cells were found in the control group. Osteonectin and osteopontin immunohistochemical detection found that the nucleus was light blue and a lot of brown particles appeared in the kytoplasm, presenting obvious strong positive, but no brown particles appeared in the control group.CONCLUSION: After combined induced culture with dexamethasone, vitamine C and β-sodiumglycerophosphate, rabbit bone marrow mensenchymal stem cells showed the morphological and biological characteristics of osteoblasts with osteoblastic activity, and were able to supply autologous seed cells for constructing in vitro tissue engineered bone in a short period of time.
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BACKGROUND: Polymorphism of exon 5 +3953C/T of interleukin-1β (IL-1β) gene is found in some studies. This gene polymorphism is related to stomach cancer, rheumatism, arthritis and other diseases. However, what is the distribution property of genotype of IL-1β gene in Guangxi Zhuang population? OBJECTIVE: To observe the distribution of the polymorphism of exon 5 +3953C/T of 1L-1β gene in Chinese Guangxi Zhuang population and Han population, and compare its distribution with Caucasians and African white people.DESIGN: Contrast observation.SETTING: Department of Histology and Embryology,Youjiang Medical College for Nationalities.PARTICIPANTS: Persons who received the health examination in the Affiliated Hospital of Youjiang Medical College for Nationalities were recruited in the experiment. Among them, 155 were Zhuang nationality, including 80 male and 75 female, aged (37) years, and 195 were Han na tionality, including 100 male and 95 female, aged (38) years. Blood relationship was not found among them,and informed consents were obtained from all the participants.METHODS: This experiment was carried out in the Experimental Center for Medical Sciences,Guangxi Medical University from June 2003 to May 2005. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used to detect the polymorphism of exon 5 +3953C/T of IL-1β gene in Guangxi Zhuang population (n=155) and Han population (n=195).Genotype and allele frequency of IL-1β gene of healthy population were directly calculated with counting method, and they were compared between Caucasians and African white people combining with literatures.MAIN OUTCOME MEASURES: Comparison of genotype frequency and allele taking frequency of IL-1β gene (+3953) among different ethnic populations.RESULTS: Subjects who received healthy examination including 155 Guangxi Zhuang nationality and 195 Chinese Han nationality participated in result analysis. ①The frequencies of genotypes and allele of IL-1β gene (+3953C/T) were close between Guangxi Zhuang population and Han nationality (Frequency of genotype CC,CT TT and allele C and T of Zhuang population was 94.8％,5.2％,0,97.4％,2.6％,respectively,and that of Han population was 93.3％,6.7％,0,96.7％,3.3％,respectively,P＞0.05).②There were significant differences in genotype distribution and allele taking frequency of IL-1β gene between Caucasians,African white people and Guangxi Zhuang population,Han population (Frequency of genotype CC,CT TT and allele C and T of Caucasians was 52.2％,43.9％,3.9％,74.1％,25.9％,respectively,and that of African white people was 60.4％,33.2％,6.4％,76.4％,23.6％, respectively, P＜0.01).CONCLUSION: The distribution of polymorphism of exon 5 +3953C/T of IL-1β gene is close between Guangxi Zhuang population and Han population,but significant difference exists in comparison with Caucasians and African white people.